Figure 3.

NKA α1 alleviated UA induced ROS production and autophagy in cultured human PTECs. The NKA α1 expression vector or siRNA was added to cells for 30 h to overexpress or inhibit NKA α1 before 48 h UA 100 μg ml⁻¹ stimulation. UA significantly increased ROS production (a, b), LDH release (c), early apoptosis (d, e) and induced autophagy, as indicated by reduced p62 (g) and an increased LC3II/LC3I ratio (h), beclin-1 (i) and LAMP2 (j) when compared with the control. The NKA α1 expression vector significantly alleviated ROS production (a, b) had a tendency to reduce early apoptosis (d, e), but had no effect on late apoptosis (d, f). The NKA α1 expression vector increased p62 expression (g) and reduced LAMP2 expression (j). The NKA α1 expression vector exerted a tendency to reduce the LC3II/LC3I ratio (h). *P<0.05 vs Cont, **P<0.01 vs Cont, #P<0.05 vs UA, ##P<0.01 vs UA.