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. 2018 Mar 16;50(3):e459. doi: 10.1038/emm.2017.299

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Copyright © 2018 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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The effect of HSP60 knockdown on mESC differentiation. (a) The morphologies of EBs that were differentiated from control shRNA or HSP60 shRNA-expressing mESCs for 3 days. (b) The mean sizes of the embryoid bodies that were differentiated from control shRNA or HSP60 shRNA-expressing mESCs for 3 days. (c, d) The effects of HSP60 knockdown on the differentiation of mESCs into three germ layers. Control shRNA or HSP60 shRNA-expressing mESCs were spontaneously differentiated through embryoid body formation and were analyzed via immunocytochemistry (c) and real-time PCR (d) for lineage markers of the three embryonic germ layers (endoderm, α-feto protein (AFP); mesoderm, brachyury; and ectoderm, nestin).