Figure 4. Hap4 is required for the intronless phenotype.
(a) Mitochondrial morphology, (b) mitochondrial volume, (c) oxygen consumption, (d) ATP levels, and (e) chronological lifespan do not differ between I0/Mss116OE and their corresponding control strains if hap4 has been deleted. Bar heights display the mean of three biological replicates (each averaged over three technical replicates). Error bars are standard error of the mean. ***p<0.001; **p<0.01; *p<0.05 (ANOVA plus post hoc). (f) Transcriptional responses in different strains where hap4 has been deleted, as measured by qPCR. Heat maps display mean values of log-fold changes observed across three biological replicates (each averaged over three technical replicates). UBC6 was used for normalization.