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. 2018 Mar 23;7:e35330. doi: 10.7554/eLife.35330

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© 2018, Rudan et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (a) qPCR measurements in strains where either the cob promoter (cobp), the cox1 promoter (cox1p) or both (pp) have been attenuated via targeted mutations. Heat maps display mean values of log-fold changes observed across three biological replicates (each averaged over three technical replicates). UBC6 was used for normalization. (b) Oxygen consumption and (c) mitochondrial morphology and (d) volume in response to promoter attenuation. Bar heights display the mean of three biological replicates (each averaged over three technical replicates). Error bars are standard error of the mean. Asterisks to indicate statistical significance are omitted for clarity. All comparisons between single- and double-promoter mutants and the corresponding parent strains are significant at p<0.001 (ANOVA plus post hoc).

Figure 5—figure supplement 1. — (a) qPCR measurements in strains where either the cob promoter (cobp), the cox1 promoter (cox1p) or both (pp) have been attenuated via targeted mutations. Heat maps display mean values of log-fold changes observed across three biological replicates (each averaged over three technical replicates). UBC6 was used for normalization. (b) Oxygen consumption and (c) mitochondrial morphology and (d) volume in response to promoter attenuation. Bar heights display the mean of three biological replicates (each averaged over three technical replicates). Error bars are standard error of the mean. Asterisks to indicate statistical significance are omitted for clarity. All comparisons between single- and double-promoter mutants and the corresponding parent strains are significant at p<0.001 (ANOVA plus post hoc).