Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Memo1 | NA | Entrez gene ID: 76890, Memo1 short isoform RefSeq ID: NM_133771.2 | |
| Genetic reagent (M. musculus) | Neurod1-Cre | The Jackson Laboratory | 028364 | |
| Genetic reagent (M. musculus) | Pcp2-Cre | The Jackson Laboratory | 004146 | |
| Genetic reagent (M. musculus) | Atoh1-Cre | The Jackson Laboratory | 011104 | |
| Genetic reagent (M. musculus) | Pabpc1-cTag | Hwang et al. (2017) | PMID: 28910620 | |
| Genetic reagent (M. musculus) | (Tg(Neurod1-Egfp-L10a) | Heiman et al. (2008) | PMID: 19013281 | |
| Genetic reagent (M. musculus) | (Tg(Pcp2-Egfp-L10a) | Heiman et al. (2008) | PMID: 19013281 | |
| Genetic reagent (M. musculus) | Memo1 knockout | Van Otterloo et al., 2016 | PMID: 26746790 | Memo1 ki/ki |
| Antibody | anti-GFP (for cTag-PAPERCLIP) | Heiman et al. (2008) | 19F7 and 19C8 | mouse monoclonal |
| Antibody | anti-Calb1 | Santa Cruz | sc-7691 | goat polyclonal, (1:250) |
| Antibody | anti-GFP (for IF) | Aves Labs | GFP-1020 | chicken polyclonal, (1:1000) |
| Antibody | anti-pH3 | Cell Signaling | 9701 | rabbit polyclonal, (1:100) |
| Antibody | Alexa 488, 555 and 647 secondaries | Thermo Fisher | (1:1000) | |
| Sequence-based reagent | miR-124 antagomir | Exiqon | 4102200–121 | mmu-miR-124–3 p inhibitor 3`-fluorescein labeled, (50 nM) |
| Sequence-based reagent | antagomir negative control | Exiqon | 199006–100 | Negative control A, (50 nM) |
| Sequence-based reagent | Memo1 miR-124 target blocker | Exiqon | 1999993 | Custom miRCURY LNA Power Inhibitor, (50 nM) |
| Sequence-based reagent | target blocker negative control | Exiqon | 199006–111 | Negative control A, (50 nM) |
| Commercial assay or kit | TruSeq RNA Library Prep Kit | Illumina | RS-122–2002 | For preparing RNA-seq libraries |
All primers are listed in Supplementary file 7.