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. 2018 Mar 26;7:e32208. doi: 10.7554/eLife.32208

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© 2018, Barrera et al

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Figure 2. — (A–B) Vessel colour changes (panels A-B) and intravascular filling defects (panel B, arrowheads) were identified during gross pathology examination (representative images of superior calotte and PO block from histology cases n. 5 and 7, respectively) N = 12. Abnormal vessels were sampled during gross pathology examination and analysed separately (see marked quadrant in panel A). (C–D) H and E staining of parasitised venules from MR cases sampled by punch biopsies from a retinal quadrant with (panel C shows the same orange vessel as in panel A) and without (panel D, case n. 15) vessel discolouration. (C) The margin of the vessel lumen has a near-complete layer of pigment-containing pRBCs (that stain less intensely pink than the adjacent non-parasitised RBC) on the endothelium. (D) Mild sequestration of pRBCs which is marked by an arrowhead. Scale bars (50 μm, (C–D).