Key resources table. Antibodies used for immunohistochemistry analysis of the clinicopathology dataset.
| Antigen | Specificity | MR feature | Manufacturer (clone); RRID* |
Host† (class) | Ag retrieval‡ | Dilution§ | Chromogen# | Staining quantification |
Ref |
|---|---|---|---|---|---|---|---|---|---|
| VEGFR1 | Retinal cell | Retinal whitening Tissue effects |
Abcam (Y103); AB_778798 |
Rb mAb (IgG) | Heat (High pH) | 1:2,000, 30 min RT | DAB | Automated | (Kaur et al., 2008a) |
| Aquaporin 4 (AQP4) | Neuroglia | Retinal whitening Tissue effects Intracellular oedema |
Abcam (EPR7040); AB_11143780 |
Rb mAb (IgG) | Heat (Low pH) | 1:500, 60 min RT | AEC | Automated | (Medana et al., 2011) |
| Glial fibrillaryacidicprotein (GFAP) | Neuroglia (late activation) |
Vessel discolouration | Dako; AB_10013482 |
Rb pAb | Proteinase K | 1:2,000, o.n. 4°C | AEC | Manual | (Hiscott et al., 1984) |
| ICAM-1 | Endothelium Neuroglia(early activation) |
Vessel discolouration | Abcam (EP1442Y); AB_870702 |
Rb mAb (IgG) | Heat (High pH) | 1:100, 30 min RT | DAB | Manual | (Lee et al., 2000) |
| CD61 | Platelets and precursors |
Retinal whitening Vessel discolouration |
Thermo Scientific; AB_929194 |
Ms mAb (IgG1) | Heat (High pH) | 1:100, 32 min RT | DAB or AEC | Manual | (White et al., 2009) |
| CD34 (II) | Endothelium | Vessel discolouration | Dako (QBEnd-10); AB_2074478 |
Ms mAb (IgG1k) | Heat (High pH) | 1:100, 30 min RT | DAB | Manual | (Kaur et al., 2008a) |
| Smooth muscle actin (SMA) |
Pericyte (venules only) |
Vessel discolouration | Dako (1A4); AB_2223500 |
Ms mAb (IgG2ak) | Heat (Low pH) | 1:2,000, o.n. 4°C | AEC | Manual | (Kaur et al., 2008b) |
| Platelet derived growth factor receptor β (PDGFRβ) |
Pericyte (signalling) |
Vessel discolouration | Abcam (Y92); AB_777165 |
Rb mAb (IgG) | Heat (Low pH) | 1:100, 30 min RT | DAB | Manual | (Armulik et al., 2005) |
*RRID: Research Resource Identifiers.
†Host: Rb = rabbit; Ms = mouse; mAb = monoclonal antibody; pAb = polyclonal antibody.
‡Ag retrieval: heat-mediated antigen retrieval was performed in high pH solution (10 mM Tris/1 mM EDTA, pH 9.0) or low pH solution (trisodium citrate 10 mM, pH 6.0). Proteinase K was from Dako (ready-to-use solution).
§Dilution and incubation time: RT = room temperature; o.n. = over night.
#Chromogen: AEC: 3-amino-9-ethylcarbazole; DAB = 3,3'-diaminobenzidine. Reported references are from main manuscript.