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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Virology. 2015 Nov 30;488:162–168. doi: 10.1016/j.virol.2015.11.012

Figure 2. Transduction efficiencies of CNV-G, PRV-G and VSV-G pseudotyped lentiviral vectors in different cell types.

Figure 2

(A–E) Transduction of adherent cells GHOST, HeLa, BHK, MDCK and N2a. Cells were exposed to MOI of 0.5, 1 or 5 for 4 hours and cultured for 72 hours before assaying for GFP expression. Percent transduction of different cell types are indicated. Transduction efficiency of CNV-G was significantly higher than VSV-G in GHOST, MDCK and N2a cells (p<0.05) (F–H) Transduction of non-adherent cells. Human lymphoid cells (Sup-T1, CEM and Jurkat cells) were transduced at MOI of 1, 5 and 10 for 4 hours and evaluated for GFP expression at 72 hours. Percent transduction of different cell types are indicated. Note that the MOIs depicted for input vector here are based on titers in HEK293T cells.