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. Author manuscript; available in PMC: 2018 Apr 13.
Published in final edited form as: Virology. 2015 Nov 30;488:162–168. doi: 10.1016/j.virol.2015.11.012

Figure 4. Resistance of CNV-G, PRV-G and VSV-G pseudotyped lentiviral vectors to freeze/thaw cycles and human serum complement inactivation.

Figure 4

(A) To determine the stability of different pseudotyped vectors during freeze-thaw cycles, the vector stocks were frozen and thawed 5 times and later titrated on HEK293T cells as described in Methods. Results presented are from three different experiments. (B) To determine the vector sensitivity to human serum complement, vector preparations were exposed to control and heat inactivated human sera for 1 hour at 37°C. Later, the exposed vector suspensions were titered on HEK293T cells. Titer values obtained from DMEM media exposure alone were set as baseline and compared with those of exposure to heat inactivated and non-inactivated sera treatment.