Table 3. Immunophenotype changes caused by K3-EDTA compared to the Na-heparin sample on day 0 in bone marrow samples.
| Cell population | Marker | Mean fluorescence intensity rates | P | |
|---|---|---|---|---|
| Na-heparin | K3-EDTA | |||
|
Granulocyte (N = 16) |
SSC | 160,346 (133,356 - 177,111) | 146,635 (129,971 - 153,327) | 0.003 |
| CD45 | 3964 (3271 – 4569) | 3113 (2696 – 4324) | 0.013 | |
| CD15 | 5743 (4329 – 6828) | 6803 (4177 – 7924) | 0.134 | |
| HLA-DR | 179 (93 – 390) | 243 (197 – 286) | 0.155 | |
| CD11b | 10,389 (7208 - 14,936) | 1990 (1593 – 3299) | < 0.001 | |
| CD13 | 1754 (1398 – 2576) | 1786 (1102 – 2288) | 0.079 | |
| CD16 | 1764 (1342 – 2119) | 1755 (1395 – 2878) | 0.836 | |
| CD10 | 1215 (977 – 1641) | 931 (801 – 1692) | 0.179 | |
| CD33 | 1334 (992 – 1670) | 1194 (878 – 1705) | 0.044 | |
| CD16% | 45.5 (40.1 - 61.3) | 47.5 (38.0 - 58.9) | 0.641 | |
| CD10% | 34.5 (29.2 - 49.5) | 34.9 (31.5 - 54.3) | 0.733 | |
|
Monocyte (N = 16) |
SSC | 75,758 (68,211 - 80,506) | 73,891 (67,578 - 80,301) | 0.125 |
| CD64 | 1330 (1140 – 2051) | 1584 (1105 – 2368) | 0.776 | |
| CD4 | 455 (369 – 543) | 496 (323 – 753) | 0.036 | |
| CD15 | 1072 (799 – 1502) | 1114 (987 – 1528) | 0.433 | |
| HLA-DR | 7178 (4578 – 7955) | 6165 (4420 – 7139) | 0.078 | |
| CD11b | 13,545 (11,385 - 20,306) | 4001 (3453 – 5080) | 0.001 | |
| CD13 | 3522 (1339 – 7092) | 3322 (1106 – 4792) | 0.031 | |
| CD14 | 889 (812 – 1482) | 971 (720 – 1254) | 0.078 | |
| CD300e | 1052 (724 – 1914) | 1070 (789 – 1804) | 0.955 | |
| CD33 | 7086 (5827 – 9495) | 6790 (5119 – 8390) | 0.078 | |
| CD14% | 55.4 (41.3 - 62.5) | 57.3 (47.8 - 62.8) | 0.842 | |
| CD300e% | 39.1 (24.2 - 58.8) | 41.5 (26.0 - 59.0) | 0.272 | |
|
Myeloblast (N = 16) |
% | 0.88 (0.62 - 2.30) | 1.06 (0.73 - 2.08) | 0.451 |
| CD45 | 3571 (3019 – 3951) | 3581 (2820 – 3938) | 0.877 | |
| CD34 | 3017 (2276 – 4231) | 2495 (1959 – 3125) | 0.008 | |
| CD117 | 2599 (2178 – 5455) | 3321 (2805 – 5956) | 0.001 | |
| Lymphoblast (N = 7) | % | 0.10 (0.06 - 0.20) | 0.08 (0.06 - 0.16) | 0.061 |
| PreB cell (N = 9) | CD10% | 0.50 (0.25 - 0.95) | 0.90 (0.40 - 1.11) | 0.172 |
|
Lymphocyte (N = 16) |
% | 9.70 (6.25 - 15.45) | 10.30 (6.03 - 14.88) | 0.82 |
| SSC | 20,777 (19,017 - 21,783) | 20,427 (18,451 - 21,907) | 0.352 | |
| CD45 | 15,833 (12,913 - 18,175) | 16,742 (13,839 - 21,026) | 0.004 | |
|
Erythroid precursor (N = 16) |
% | 18.80 (10.33 - 31.25) | 19.40 (11.83 - 31.25) | 0.776 |
| CD71 | 6158 (3767 – 8283) | 3564 (2201 – 5261) | 0.003 | |
| CD71rCV | 79.5 (53.8 - 112.3) | 102.7 (81.0 - 117.7) | 0.049 | |
|
Plasma cell (N = 15) |
% | 0.2 (0.2 - 0.5) | 0.5 (0.3 - 0.9) | 0.005 |
| CD38 | 29,173 (25,738 - 32,208) | 35,393 (29,221 - 38,793) | 0.002 | |
| SSC - side scatter. Mean fluorescence intensity values and percentages (%) are presented as median and interquartile range. The number of cases by cell population is different because certain cell types were not detectable in all samples (sensitivity: 0.03). The groups (K3-EDTA and Na-heparin) were compared by Wilcoxon signed-rank test. P < 0.05 values were considered statistically significant. | ||||