Table 4. Time-dependent immunophenotype changes in bone marrow samples collected with K3-EDTA and Na-heparin.
| Tube type | Cell population | Marker | Mean fluorescence intensity rates | P1 | P2 | ||
|---|---|---|---|---|---|---|---|
| day 0 | day 1 | day 2 | |||||
| K3-EDTA |
Granulocyte (N = 23) |
SSC | 153,327 (138,520 – 176,341) | 149,831 (134,405 – 172,463) | 124,668 (110,787 - 148,576) | > 0.999 | < 0.001 |
| CD45 | 3000 (2739 – 3753) | 4141 (3313 – 4472) | 3610 (2787 – 3972) | 0.037 | > 0.999 | ||
| CD15 | 3900 (2199 – 7126) | 4681 (2203 – 6913) | 3734 (2473 – 6290) | > 0.999 | 0.554 | ||
| HLA-DR | 235 (185 – 285) | 269 (233 – 341) | 324 (261 – 452) | 0.037 | < 0.001 | ||
| CD11b | 2795 (1709 – 3613) | 1566 (887 – 2406) | 642 (436 – 1082) | 0.117 | < 0.001 | ||
| CD13 | 1845 (1185 – 2308) | 2538 (1875 – 3073) | 2607 (2162 – 3296) | 0.006 | 0.001 | ||
| CD16 | 2179 (1491 – 2930) | 2102 (1750 – 3000) | 1780 (1003 - 2727) | 0.554 | 0.554 | ||
| CD10 | 1183 (847 – 1627) | 1178 (1001 – 1768) | 976 (807 - 1285) | 0.231 | 0.231 | ||
| CD33 | 1212 (815 – 1717) | 1349 (917 – 1773) | 1188 (754 – 1678) | 0.055 | > 0.999 | ||
| CD16% | 49.0 (42.0 - 60.8) | 51.0 (43.0 - 56.2) | 50.0 (40.7 - 59.0) | 0.029 | 0.715 | ||
| CD10% | 38.1 (31.0 - 47.0) | 41.0 (34.4 - 49.5) | 38.4 (31.7 - 45.0) | 0.081 | > 0.999 | ||
|
Monocyte (N = 23) |
SSC | 79,099 (71,934 - 83,805) | 68,674 (64,864 - 75,911) | 62,401 (60,160 - 67,200) | 0.117 | < 0.001 | |
| CD64 | 1596 (1107 – 1886) | 1441 (1052 – 1757) | 973 (732 – 1544) | 0.117 | < 0.001 | ||
| CD4 | 579 (422 – 691) | 512 (341 – 712) | 418 (324 – 552) | > 0.999 | 0.024 | ||
| CD15 | 967 (280 – 1290) | 632 (281 – 836) | 564 (309 – 924) | 0.081 | 0.024 | ||
| HLA-DR | 6320 (5487 – 7790) | 5779 (4291 – 10,731) | 5109 (3969 – 7598) | > 0.999 | 0.315 | ||
| CD11b | 4700 (3712 – 6538) | 716 (342 – 1026) | 325 (209 – 406) | < 0.001 | < 0.001 | ||
| CD13 | 4190 (1403 – 6409) | 2873 (1156 – 5090) | 2928 (2035 – 4448) | 0.006 | 0.024 | ||
| CD14 | 1121 (777 – 1571) | 777 (627 – 1023) | 556 (377 – 718) | 0.037 | < 0.001 | ||
| CD300e | 1423 (1055 – 1787) | 767 (556 – 1263) | 523 (379 – 925) | 0.081 | < 0.001 | ||
| CD33 | 6747 (5015 – 8633) | 5270 (3750 – 8869) | 4045 (3101 – 5355) | > 0.999 | < 0.001 | ||
| CD14% | 60.0 (50.0 - 65.8) | 53.0 (40.0 - 59.0) | 25.0 (17.0 - 44.0) | 0.231 | < 0.001 | ||
| CD300e% | 43.4 (26.5 - 56.0) | 31.3 (25.0 - 47.0) | 27.7 (13.1 - 36.0) | 0.037 | < 0.001 | ||
|
Myeloblast (N = 23) |
% | 0.92 (0.73 - 1.47) | 0.81 (0.54 - 1.35) | 0.76 (0.57 - 0.97) | 0.196 | 0.008 | |
| CD45 | 3555 (2383 – 3838) | 3242 (2286 – 3992) | 3034 (2636 – 4113) | 0.421 | 0.315 | ||
| CD34 | 3023 (2262 – 3709) | 2976 (1987 – 3484) | 2318 (1829 – 3860) | 0.554 | 0.231 | ||
| CD117 | 3148 (2781 – 3745) | 2411 (2128 – 3523) | 1876 (1532 – 2592) | 0.006 | < 0.001 | ||
|
Lymphoblast (N = 12) |
% | 0.17 (0.06 - 0.23) | 0.15 (0.05 - 0.17) | 0.09 (0.06 - 0.16) | 0.554 | 0.459 | |
|
PreB cell (N = 17) |
CD10% | 0.90 (0.23 - 1.64) | 0.50 (0.19 - 1.02) | 0.40 (0.16 - 0.70) | 0.215 | < 0.001 | |
|
Lymphocyte (N = 23) |
% | 10.9 (6.6 - 16.2) | 10.0 (5.7 - 14.7) | 8.3 (4.9 - 12.8) | 0.554 | < 0.001 | |
| SSC | 21,410 (19,704 - 22,477) | 19,611 (17,655 - 20,994) | 18,366 (16,682 - 19,817) | 0.055 | < 0.001 | ||
| CD45 | 15,962 (14,646 - 19,342) | 14,898 (12,197 - 17,206) | 13,087 (9894 - 16,167) | 0.081 | < 0.001 | ||
|
Erythroid precursor (N = 23) |
% | 14.3 (9.2 - 27.7) | 14.1 (10.0 - 29.0) | 13.9 (9.4 - 28.0) | 0.715 | > 0.999 | |
| CD71 | 4896 (2448 – 6486) | 3164 (1888 – 4449) | 2486 (1792 – 3552) | 0.315 | 0.015 | ||
| CD71rCV | 95 (80 - 108.9) | 88.9 (68.7 - 113.2) | 84.4 (63 - 103.4) | > 0.999 | > 0.999 | ||
|
Plasma cell (N = 22) |
% | 0.50 (0.32 - 0.70) | 0.50 (0.30 - 0.63) | 0.46 (0.30 - 0.74) | 0.457 | > 0.999 | |
| CD38 | 36,019 (31,070 - 39,660) | 28,946 (24,769 - 33,504) | 25,467 (21,210 - 31,042) | 0.001 | < 0.001 | ||
| Na - heparin |
Granulocyte (N = 16) |
SSC | 160,346 (133,356 – 177,111) | 146,104 (125,819 – 176,537) | 129,252 (109,198 - 163,244) | > 0.999 | 0.004 |
| CD45 | 3964 (3271 – 4569) | 4680 (3576 – 5277) | 4039 (3437 – 4859) | 0.335 | > 0.999 | ||
| CD15 | 5743 (4329 – 6828) | 5245 (3720 – 6371) | 4398 (2832 – 5335) | 0.867 | 0.024 | ||
| HLA-DR | 179 (93 – 390) | 197 (118 - 302) | 290 (171 – 492) | > 0.999 | 0.024 | ||
| CD11b | 10,389 (7208 – 14,936) | 11,877 (9311 – 16,119) | 10,517 (7569 - 13,451) | 0.867 | > 0.999 | ||
| CD13 | 1754 (1398 - 2576) | 2218 (1641 - 2680) | 2111 (1869 - 3250) | 0.648 | 0.008 | ||
| CD16 | 1764 (1342 – 2119) | 1939 (1229 – 2618) | 1467 (643 – 2142) | > 0.999 | 0.102 | ||
| CD10 | 1215 (977 – 1641) | 1222 (924 – 1805) | 997 (667 - 1341) | 0.867 | 0.335 | ||
| CD33 | 1334 (992 – 1670) | 1531 (1071 – 1891) | 1403 (932 – 1827) | 0.472 | > 0.999 | ||
| CD16% | 46 (40 – 61) | 47 (38 – 59) | 40 (31 – 52) | > 0.999 | 0.014 | ||
| CD10% | 35 (29 – 50) | 37 (30 – 49) | 36 (25 – 46) | > 0.999 | 0.752 | ||
|
Monocyte (N = 16) |
SSC | 75,758 (68,211 - 80,506) | 76,178 (70,576 - 81,870) | 72,624 (66,219 - 81,805) | > 0.999 | > 0.999 | |
| CD64 | 1330 (1140 – 2051) | 1434 (898 - 1966) | 1369 (808 – 1803) | 0.156 | < 0.001 | ||
| CD4 | 455 (369 – 543) | 344 (276 - 590) | 261 (208 – 421) | 0.335 | < 0.001 | ||
| CD15 | 1072 (799 - 1502) | 751 (593 - 1249) | 681 (496 – 829) | 0.024 | < 0.001 | ||
| HLA-DR | 7178 (4578 - 7955) | 8003 (4743 – 11,516) | 7402 (5690 – 8140) | 0.472 | > 0.999 | ||
| CD11b | 13,545 (11,385 - 20,306) | 10,174 (7165 – 12,797) | 7428 (4317 – 12,032) | 0.232 | < 0.001 | ||
| CD13 | 3522 (1339 – 7092) | 2859 (1195 – 5494) | 2559 (1530 - 4105) | 0.335 | 0.867 | ||
| CD14 | 889 (812 – 1482) | 878 (650 – 1008) | 637 (545 – 907) | > 0.999 | 0.014 | ||
| CD300e | 1052 (724 – 1914) | 1116 (708 – 1528) | 783 (644 – 1273) | 0.867 | > 0.999 | ||
| CD33 | 7086 (5827 – 9495) | 5658 (4675 – 8438) | 4443 (3817 – 5897) | 0.232 | < 0.001 | ||
| CD14% | 55.4 (41.3 - 62.5) | 50.5 (38.8 - 59.8) | 36.5 (22.3 - 45.5) | > 0.999 | < 0.001 | ||
| CD300e% | 39.1 (24.2 - 58.8) | 41.9 (24.5- 50.8) | 32.0 (24.0 - 52.3) | > 0.999 | > 0.999 | ||
|
Myeloblast (N = 16) |
% | 0.88 (0.62 - 2.30) | 0.74 (0.50 - 1.95) | 0.73 (0.42 - 2.12) | 0.279 | 0.065 | |
| CD45 | 3571 (3019 – 3951) | 3554 (2594 – 4571) | 3484 (2780 – 4096) | > 0.999 | > 0.999 | ||
| CD34 | 3017 (2276 – 4231) | 2624 (1535 – 3053) | 2137 (1680 – 2812) | 0.335 | 0.102 | ||
| CD117 | 2599 (2178 – 5455) | 1973 (1355 – 3308) | 1524 (1186 – 2572) | 0.004 | < 0.001 | ||
|
Lymphoblast (N = 8) |
% | 0.09 (0.05 - 0.18) | 0.07 (0.03 - 0.14) | 0.07 (0.02 - 0.10) | > 0.999 | 0.508 | |
|
PreB cell (N = 9) |
CD10% | 0.50 (0.25 - 0.95) | 0.52 (0.25 - 0.85) | 0.30 (0.17 - 0.70) | > 0.999 | 0.001 | |
|
Lymphocyte (N = 16) |
% | 9.7 (6.3 - 15.5) | 7.7 (4.5 - 13.2) | 6.9 (3.3 - 10.0) | 0.279 | 0.001 | |
| SSC | 20,777 (19,017 - 21,783) | 17,295 (15,546 - 20,467) | 17,232 (15,318 - 18,176) | 0.024 | < 0.001 | ||
| CD45 | 15,833 (12,913 - 18,175) | 12,204 (9853 - 17,172) | 9948 (8465 -14,071) | 0.472 | 0.002 | ||
|
Erythroid precursor (N = 16) |
% | 18.8 (10.3 - 31.3) | 20.1 (10.8 - 31.5) | 19.2 (10.4 - 33.8) | > 0.999 | 0.867 | |
| CD71 | 6158 (3767 – 8283) | 4367 (2268 - 7238) | 3479 (2233 – 7007) | 0.156 | 0.002 | ||
| CD71rCV | 79.5 (53.8 - 112.3) | 82.1 (67.5 - 114.3) | 80.9 (61.3 - 107.3) | > 0.999 | > 0.999 | ||
|
Plasma cell (N = 15) |
% | 0.20 (0.20 - 0.50) | 0.20 (0.10 - 0.50) | 0.30 (0.10 - 0.50) | 0.166 | > 0.999 | |
| CD38 | 29,173 (25,738 - 32,208) | 19,312 (17,781 - 27,495) | 17,022 (14,953 - 18,877) | 0.032 | < 0.001 | ||
| SSC - side scatter. Mean fluorescence intensity values and percentages (%) are presented as median and interquartile range. P1 - comparison of day 0 and day 1 results. P2 - comparison of day 0 and day 2 results. The number of cases by cell population is different because certain cell types were not detectable in all samples (sensitivity: 0.03). The groups were compared by Friedman test. Dunn’s multiple comparison test was applied as post hoc test. P < 0.05 values were considered statistically significant. | |||||||