Figure 5.

Kinetics of saturation of phosphorylated maize leaf PEPC by Glc6P (▪), Ala (□), or Gly (●) and by Gly in the presence of 10 mm Glc6P (○). A was in the absence and B was in the presence of 20% (v/v) glycerol. The insets show the saturation by Glc6P in a small scale. Assays were carried out in the presence of 20 mm malate at 3 mm total PEP, 0.1 mm bicarbonate, and 0.4 mm free Mg²⁺. The points are the experimental data. The lines are the result of the best fit of the experimental data to Equation 12 or 13 as appropriate.