. 2000 May;123(1):149–160. doi: 10.1104/pp.123.1.149

Table II.

Apparent kinetic parameters^a of the nonphosphorylated and phosphorylated forms of maize leaf PEPC at pH 7.3, 0.4 mm free Mg²⁺, and 0.1 mm bicarbonate

Parameter	Nonphosphorylated	Phosphorylated
Saturation by total PEP
V_max (units mg⁻¹ protein)	13.4 ± 0.3	11.0 ± 0.5
S_0.5 (mm)	12.3 ± 0.3	9.1 ± 0.6
n	2.1 ± 0.1	2.0 ± 0.2
Saturation by Glc6P^a
v_amax (units mg⁻¹ protein)	5.5 ± 0.2	5.8 ± 0.1
A_0.5 (mm)	3.9 ± 0.3	2.0 ± 0.1
n	1.7 ± 0.1	1.6 ± 0.1
Saturation by Gly^a
v_amax (units mg⁻¹ protein)	16.1 ± 0.5	11.5 ± 0.2
A_0.5 (mm)	14.5 ± 1.6	3.1 ± 0.3
n	1.0 ± 0.1	0.9 ± 0.1
Saturation by malate^a
I₅₀ (mm)	0.27 ± 0.01	0.72 ± 0.01

Values ± sd were estimated by the best fit to Equations 2, 5, and 6 for saturation by total PEP, by Glc6P or Gly, and by malate, respectively.

The apparent kinetic parameters were determined at 3 mm total PEP.