Figure 2.

LC₅₀ value of 1a decreases with increasing drug exposure time. (A) Parasite growth was measured at 72 h by the SYBR Green assay in cultures where 1a was washed out at 6 or 12 h, and parasites were returned to culture to complete 72 h. A representative growth inhibition curve at 72 h from parasites continuously exposed to 1a is also shown. (B) 1a becomes cytocidal at 18 h drug bolus time when parasites reach late trophozoite/early schizont stages. The reported values represent averages and SEM of at least three independent assays.