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. Author manuscript; available in PMC: 2019 Apr 13.
Published in final edited form as: ACS Infect Dis. 2017 Nov 7;4(4):549–559. doi: 10.1021/acsinfecdis.7b00159

Figure 3.

Figure 3

Highly synchronous parasites were recovered at different stages of P. falciparum intraerythrocytic cycle (12 hpi: rings; 19 hpi: trophozoites; 33 hpi: schizonts), and the presence of the MEP pathway intermediates was assessed by quantitative LC-MS/MS where only CDP-ME, MEcPP, and IPP were detected. The levels of all three detected intermediates increased markedly between trophozoite (19 hpi) and schizont (33 hpi) stages. The response of each detected metabolite is expressed as the mean and SEM of two biological replicates. 1: 1-Deoxy-D-xylulose-5-phosphate (DOXP) reductoisomerase; 2: IspD; 3: CDP-ME kinase; 4: 2C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcPP) synthase; 5: 4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP) synthase; 6: HMBPP reductase.