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Effect of plasma membrane Ca2+ channel blocker verapamil on heat (A) or cold shock (B) induced changes in [Ca2+]i. Cells were preincubated with 200 μm verapamil for 30 min prior to heat or cold shock treatment (▵). These experiments were repeated six times and the traces represented have been chosen to best represent the average result. The vertical arrows indicate the injection of hot (A) or cold (B) water. □, Control.
