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. 2018 Apr 13;8:5975. doi: 10.1038/s41598-018-24199-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype of neonatal TUB6 mutants. Flow cytometric analysis of indicated cell populations from 2–5-day-old pups: splenocyte frequencies (a) and CD8SP and CD4SP thymocytes (b). a and b show pooled data from 3 litters analysed on 3 different days (n = 5 WT, 4 het, 4 hom). (c) Analysis of thymic stroma: cortical thymic epithelial cells (cTECs: Epcam⁺Ly51⁺) and medullary thymic epithelial cells (mTECs: Epcam⁺Ly51⁻), gated on CD45⁻ cells (n = 2), shown as percentages of CD45⁻ cells or as total cell counts. (a–c) For flow cytometry dot plots see Supplementary Fig. S3.