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. 2018 Apr 13;8:5984. doi: 10.1038/s41598-018-24295-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterial cell multiplication and EPS synthesis by confocal laser scanning microscopy (CLSM). (A) The three-dimensional reconstruction of biofilms. Reconstruction of the biofilms was performed with IMARIS 7.0. Bacterial cells were labelled with the SYTO 9 green fluorescent dye (left column), and EPS was labelled with the Alexa Fluor 647 red fluorescent dye (middle column). (B) The EPS and bacteria distributions on the reconstructed biofilm. (C) The EPS/bacteria ratio. The asterisks indicate significant differences compared to the parental S. mutans strain group. The error bars represent the standard deviation (SD). *P < 0.05.

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