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. 2018 Apr 13;8:5979. doi: 10.1038/s41598-018-24332-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Variance accounted for (VAF) as a determination of the number of extracted muscle synergies under each running condition. Iterative analysis was performed by varying the number of synergies between one and ten, and the least number of synergies that accounted for >90% VAF in each subject was identified (gray narrow lines). Colored bold lines indicate mean VAFs across subjects, with 90% VAF shown as a solid black line.