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. 2018 Apr 13;9:1462. doi: 10.1038/s41467-018-03949-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Horizontal transmission of pKSR100. Three whole-genome phylogenies for MSMA S. flexneri 3a, and S. flexneri 2a and S. sonnei are shown leftmost with successful and MSMA sublineages (respectively) highlighted in colour. The plasmid tree is a phylogeny of pKSR100 constructed from isolates containing pKSR100. Scale bars in single nucleotide polymorphisms (SNPs) are shown below each phylogeny. Individual isolate genome positions are joined to their plasmid phylogenetic position by intervening coloured lines. Two examples of horizontal transmission are indicated by arrows coloured according to the species. A time-series animation of this figure showing change over time and a static explanatory model are available elsewhere (see: Supplementary Movie 1 and Supplementary Fig. 7)