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. 2018 Apr 13;9:1466. doi: 10.1038/s41467-018-03731-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Cas9 expression in the brain of TVA/Cas9 mouse strains. a, b Immunofluorescence staining performed on brain sections of four-week-old Ntv-a; Nes-Cre; LSL-Cas9 and Gtv-a; hGFAP-Cre; LSL-Cas9 mice with antibody against EGFP (Cas9), NESTIN and GFAP. CAS9 is widely expressed in the whole brain and co-localize with NESTIN and GFAP in the subventricular zone. Left panels: whole brain section; right panels: higher magnification of the left panel inset. Scale bars: left panels, 500 μm; right panels, 100 μm. LV lateral ventricle