Table 2.
S. pyogenes proteins present in human fluid samples. The identification of each protein was determined by SEQUEST and was based on matching mass spectra of at least 2 different peptides for each protein. All proteins were matched to those from an emm1 strain, although (with the exception of inhibitor of complement protein and hypothetical protein M5005_Spy0455), others were also matched to most, and often all, other emm types examined, as detailed in Supplementary Table S1. The resultant probability calculation for each protein is given along with the number of peptides found. The peptide identifications were compared with results obtained from proteomic analysis of supernatant samples and in all cases, except for hypothetical protein M5005_Spy0455, between 1 and 9 peptides matching both supernatant and clinical fluid sample were found, providing additional confidence in these identifications.
| Protein name | Locus tag | Fluid | Protein probability | No. of peptides | Common peptides |
|---|---|---|---|---|---|
| 30S ribosomal protein S2 | M5005_Spy1780 | F8 | 2.5E-05 | 3 | 3/3 |
| elongation factor G | M5005_Spy0232 | F8 | 3.6E-06 | 2 | 2/2 |
| elongation factor Ts | M5005_Spy1781 | F8 | 4.9E-04 | 2 | 1/2 |
| elongation factor Tu | M5005_Spy0508 | F8 | 3.4E-12 | 9 | 9/10 |
| exotoxin B/SpeB | M5005_Spy1735 | F2 | 3.6E-08 | 3 | 3/3 |
| fructose-bisphosphate aldolase | M5005_Spy1607 | F8 | 4.9E-05 | 3 | 3/3 |
| Gls24 family general stress protein | M5005_Spy0973 | F8 | 1.5E-09 | 10 | 2/10 |
| glyceraldehyde-3-phosphate dehydrogenase | M5005_Spy0233 | F5 | 9.6E-14 | 2 | 2/2 |
| glyceraldehyde-3-phosphate dehydrogenase | M5005_Spy0233 | F8 | 1.4E-13 | 4 | 4/4 |
| histidine triad protein | M5005_Spy1710 | F8 | 1.6E-07 | 3 | 2/3 |
| hypothetical protein | M5005_Spy0269 | F8 | 1.2E-07 | 2 | 2/2 |
| hypothetical protein | M5005_Spy0455 | F4 | 3.1E-04 | 2 | 0/2 |
| hypothetical protein | M5005_Spy0455 | F7 | 6.1E-04 | 2 | 0/2 |
| inhibitor of complement protein/SIC | M5005_Spy1718 | F8 | 1.4E-09 | 3 | 3/3 |
| manganese-binding protein | M5005_Spy0368 | F8 | 4.0E-07 | 2 | 2/2 |
| manganese-dependent inorganic pyrophosphatase | M5005_Spy0319 | F8 | 8.1E-09 | 2 | 2/2 |
| molecular chaperone GroEL | M5005_Spy1761 | F8 | 2.2E-12 | 8 | 8/8 |
| N-acetylmannosamine kinase | M5005_Spy0218 | F17 | 6.0E-04 | 2 | 2/2 |
| phosphoglycerate kinase | M5005_Spy1599 | F8 | 2.0E-07 | 7 | 7/7 |
| phosphopyruvate hydratase | M5005_Spy0556 | F5 | 2.0E-08 | 4 | 4/4 |
| phosphopyruvate hydratase | M5005_Spy0556 | F8 | 1.1E-11 | 10 | 10/10 |
| trigger factor | M5005_Spy1612 | F8 | 3.7E-06 | 2 | 1/2 |