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. 2018 Apr 13;8:5958. doi: 10.1038/s41598-018-24220-6

Figure 4.

Figure 4

rHhip effect in mECs. (a) rHhip dose-dependent effect analyzed by DHE staining, TUNEL, α-SMA- and Nox4-IF staining (scale bar, 50 µm). (b) rHhip dose-dependent effect on a variety of proteins expression analyzed by WB. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; NS, non-significant vs. mECs cultured in 5 mM glucose (100%) without rHhip (0 ng/ml); Values represent the mean ± SEM. (c) The inhibitory effect of GKT137831 (10 µM) on DHE staining with or without rHhip (5 ng/ml) (scale bar, 50 µm). **P ≤ 0.01; ***P ≤ 0.001; NS, non-significant vs. mECs cultured in 5 mM glucose (100%) without rHhip (0 ng/ml).