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. 2018 Apr 13;8:5949. doi: 10.1038/s41598-018-24503-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of siRNA/shRNA efficiency after hTERT gene knockdown in HNSCC cell lines. (A) hTERT gene expression at the transcriptional level measured by qPCR; (B) hTERT gene expression at the protein level (siRNA) with Western blot analysis. Semi-quantitative analysis of Western blot results using ImageJ software; (C) hTERT gene expression at the protein level (shRNA) by immunofluorescence staining, objective magnification 20x; (D) relative telomere length analysis by qPCR; (E) cell proliferation microscopic observation, objective magnification 20x; (F) cell migration wound healing assay, objective magnification 20x. CTRL – control cells transfected with non-specific siRNA/cells transduced with control lentiviral vector; siTERT – cells transfected with hTERT gene-targeted siRNA on day 3^rd and 7^th; shTERT – cells transduced with lentiviral vectors containing shRNA. Data are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 with comparisons indicated by lines.