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. 2018 Apr 13;8:5949. doi: 10.1038/s41598-018-24503-y

Figure 2.

Figure 2

Cell cycle arrest analysis after silencing of hTERT gene expression. (A) Flow cytometry analysis; (B) Analysis of cell cycle arrest-related genes expression at the transcriptional level by qPCR. CTRL – control cells transfected with non-specific siRNA/cells transduced with control lentiviral vector; siTERT – cells transfected with hTERT gene-targeted siRNA on day 3rd and 7th; shTERT – cells transduced with lentiviral vectors containing shRNA on day 10th. Data are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 with comparisons indicated by lines.