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. 2018 Apr 13;8:5949. doi: 10.1038/s41598-018-24503-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The impact of hTERT gene downregulation on Double-Strand Breaks level after ionizing radiation alone in HNSCC cell lines. (A) cytometric analysis of Double-Strand Breaks marker - γH2AX – FaDu cells; (B) cytometric analysis of Double-Strand Breaks marker - γH2AX – H103 cells. CTRL – control cells transfected with non-specific siRNA/cells transduced with control lentiviral vector; siTERT – cells transfected with hTERT gene-targeted siRNA on day 3^rd and 7^th; shTERT – cells transduced with lentiviral vectors containing shRNA on day 10^th. Data are presented as the median ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 with comparisons indicated by lines.