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. 2018 Apr 13;8:5949. doi: 10.1038/s41598-018-24503-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA Damage Repair mechanisms activation after hTERT knockdown (siRNA) in HNSCC cell lines. (A) Analysis of DDR-related genes expression at the transcriptional level in FaDu cells by qPCR; (B) Analysis of DDR-related genes expression at the transcriptional level in H103 cells by qPCR; (C) Analysis of DDR-related genes expression at the protein level by Western blot analysis. Semi-quantitative analysis of Western blot results using ImageJ software. CTRL – control cells transfected with non-specific siRNA; d3/d7 - cells transfected with hTERT gene-targeted siRNA on day 3^rd and 7^th respectively. Data are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 with comparisons indicated by lines.