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. 2018 Apr 13;8:5969. doi: 10.1038/s41598-018-24320-3

Figure 1.

Figure 1

Binding of apoE4 to previously “blocked” ELISA plates. Recombinant apoE4 (dark gray bars), plasma from an APOE ε3/ε4 donor (light grey bars) or PBS (open bars) was allowed to interact with ELISA plate wells previously treated with different suitable blocking solutions: PBS (PBS with no blocking solution), BSA (0.25% BSA solution in 15 mM borate buffer containing 100 mM NaCl, pH 8.5), Superblock (Superblock T20 (ThermoFisher Scientific)), Polysorbate 20 (1% polysorbate 20 in PBS), Triton X-100 (1% Triton X-100 in PBS), Skim milk (5% skim milk in PBS containing 0.1% polysorbate 20), ODGP (8 mM Octyl b-D-glucopyranoside in PBS. Error bars represent the standard deviation of duplicated measures performed in two independent experiments.