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. 2018 Apr 13;8:5969. doi: 10.1038/s41598-018-24320-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stability of the binding of apoE to polystyrene as a function of pH, salt and detergent concentrations. Analysis of the stability of the binding of plasma apoE to polystyrene surfaces was challenged by varying pH (A), increasing salt concentrations (B) or detergent (0–0.5% polysorbate 20 or Triton X-100) (C) and detected by polyclonal pan-apoE antibody. Light and dark gray bars represent samples analyzed in wells blocked with a BSA-based or Superblock blocking solutions, respectively. Error bars represent the standard deviation of duplicated measures.