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. 2018 Apr 13;6(7):e13666. doi: 10.14814/phy2.13666

Figure 9.

Figure 9

Known ester derivatization reactions with acetate. Reaction 1 shows acetate derivatization to benzyl acetate using benzyldimethylammonium hydroxide as an ester derivatizing agent.(Richards et al. 1975) Reaction 2 shows acetate derivatization to propyl acetate using methylchloroformate as a derivatizing agent, followed by a subsequent reaction with propyl alcohol.(Tumanov et al. 2016) Any amino acids, carboxylic acids or nucleophiles remaining in samples are capable of reacting with derivatizing reagents and forming side products, drastically reducing the apparent concentration of SCFA and or leading to wide ranges for reported values. BDMPH, (benzyldimethylphenylammonium hydroxide; MCF, methylchloroformate.