FIG 1.

Schematic representation of constructs used in this study. (A to D) PPV and PPV mutants (A), LONG and LONG mutants (B), LONG deletions (C), and other plasmids (D). The constitutive 35S promoter from Cauliflower mosaic virus is shown as a black arrow. Gray boxes represent cistrons of the indicated viral proteins. A green bar between viral cistrons denotes the presence of the GFP reporter. The presence of both 5′ and 3′ viral noncoding regions and poly(A) are indicated (5′, 3′, and polyA, respectively). P3 coding sequence carries intron I from the potato ST-LS-1 gene to avoid toxicity of P3 in bacteria and increase plasmid stability.