FIG 3.

P3-coding sequences contribute to CP stability. All panels (A, B, and C) show analyses of the stability of CP produced by agroinfiltration of N. benthamiana leaves with different deletion mutants of pLONG coexpressed with the indicated constructs. Samples were collected at 5 days postagroinfiltration. Extracts prepared in SDS buffer (SDS) and the time, in minutes, elapsed after extraction with phosphate buffer (0′ and 30′) are indicated. Protein accumulation was assessed by immunoblot analysis with polyclonal serum specific for PPV CP and a monoclonal antibody specific for GFP. Membranes stained with Ponceau red showing the RubisCO large subunit were included as loading controls.