FIG 3.

uc002yug.2 expression is upregulated after HIV-1 infection or reactivation. (A and B) HIV-1 infection upregulates uc002yug.2 expression in Jurkat cells. (A) Differences in expression levels of uc002yug.2 between the mock- and HIV-1 NL4-3-infected Jurkat cells were detected by qRT-PCR. (B) Levels of CAp24 from HIV-1 NL4-3 were measured by immunoblotting. (C to F) Reactivation of HIV-1 increases uc002yug.2. HIV-1 latently infected J-Lat 6.3 and ACH-2 cells were utilized for measuring the change of uc002yug.2 during virus reactivation. (C) J-Lat 6.3 cells were treated or not with PMA (1 μM) for 48 h. Differences in expression levels of uc002yug.2, RUNX1a, and RUNX1b and -1c between the mock-infected and reactivated cells were detected by qRT-PCR. (D) HIV-1 reactivation of J-Lat 6.3 cells was measured by detecting GFP-positive cells by flow cytometry. (E) ACH-2 cells were treated or not with PMA (1 μM) for 48 h. Differences in expression levels of uc002yug.2, RUNX1a, and RUNX1b and -1c between the mock-infected and reactivated cells were detected by qRT-PCR. (F) HIV-1 reactivation of ACH-2 cells was measured by immunoblotting with anti-CAp24. (G and H) Low uc002yug.2 level maintains HIV-1 latency. (G) ACH-2 cells are HIV-1 latently infected CEM cells. The uc002yug.2 expression in acutely or latently HIV-1-infected CEM cells was detected using a qRT-PCR assay. (H) J-Lat 6.3 cells are HIV-1 latently infected Jurkat cells. The uc002yug.2 expression in acutely or latently HIV-1-infected Jurkat cells was detected using a qRT-PCR assay. Results are representative of those from three independent repeats. The qRT-PCR results in panels A, C, E, G, and H are represented with corresponding values, with the mock-infected cells set at 100%. Data are presented as means ± SDs.