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. 2018 Apr 13;92(9):e01844-17. doi: 10.1128/JVI.01844-17

TABLE 2.

Primers used for plasmid construction in this study

Primer name Primer direction Sequence (5′–3′)
uc002yug.2-F Forward GCGTCGACAAGCAGAGCTCCATCAGTC
uc002yug.2-R Reverse CGGGATCCGAGATGGAGTCTCATTCCATTTCCCAG
uc002yug.2-lenti-F Forward CCCTCGAGAAGCAGAGCTCCATCAGTC
uc002yug.2-lenti-R Reverse CGGGATCCGAGATGGAGTCTCATTCCATTTCCCAGGCTGAAGTGCAAC
uc002yug.2-d275nt-F Forward ATTTCAAGATGGGTCAGGATCCAGATCTGCTGTGCC
uc002yug.2-d275nt-R Reverse CAGCAGATCTGGATCCTGACCCATCTTGAAATTC
uc002yug.2sh-F Forward CCGGGCTGGTAGATGCCATTTGTCTCGAGACAAATGGCATCTACCAGCTTTTTG
uc002yug.2sh-R Reverse AATTCAAAAAGCTGGTAGATGCCATTTGTCTCGAGACAAATGGCATCTACCAGC
RUNX1a-Myc-F Forward GCGTCGACACCATGCGTATCCCCGTAGATG
RUNX1a-Myc-R Reverse CGGGATCCTTAAAGATCTTCTTCTGATATGAGTTTTTGTTCTTAACATCTCCAGGGTGCTG
RUNX1b-Myc-F Forward AAATATGCGGCCGCACCATGCGTATCCCCGTAGATGC
RUNX1b-Myc-R Reverse GCTCTAGATTAAAGATCTTCTTCTGATATGAGTTTTTGTTCTCAGTAGGGCCTCCACACG
CBFβ-HA-F Forward GCTAGCAAGATGCCGCGCGTCGTG
CBFβ-HA-R Reverse AAGCTTACTACGCGTAATCTGGGACGTCGTAAGGGTAGGGTCTTGTTGTCTTCTTGCC
HIV-LTR-F Forward CGGGGTACCTGGAAGGGCTAATTTGGTCCCA
HIV-LTR-R Reverse CGCGGATCCTGCTAGAGATTTTCCACACTGA
Tat-exon1-F Forward GCGTCGACACCATGGAGCCAGTAGATCCTAGACT
Tat-exon1-R Reverse GGGATTGGGAGGTGGGTTGCTTTGATAGAGAAACTTGATG
Tat-exon2-F Forward TTCTCTATCAAAGCAACCCACCTCCCAATCCCGAGGGGAC
Tat-exon2-R Reverse CGGGATCCTTACGCGTAATCTGGGACGTCGTAAGGGTAAATCGAATGGATCTGTCTCTG
dTAR-F Forward GGGTCTCTCTGGTTAGTCTGGCTAACTAGGGAACCC
dTAR-R Reverse TCCCTAGTTAGCCAGACTAACCAGAGAGACCCAGTAC
RUNXMut-F Forward ATCCTTGATCTGTGGATCTCACACACACAAGGCTACTTCC
RUNXMut-R Reverse GGAAGTAGCCTTGTGTGTGTGAGATCCACAGATCAAGGAT