FIG 3.

PRRSV nsp12 induces KPNA6 elevation. (A) Screening of PRRSV nsp to identify the viral protein that is responsible for the elevation of KPNA6. Empty vector (EV) was included as a control. At 36 h posttransfection, HEK293 cells were harvested for WB. The lower panel of images show the bands of PRRSV nsp. The relative levels of KPNA6 protein are shown below the images. Molecular mass markers are denoted on left of the images in kilodaltons. (B) Higher KPNA6 level in the nsp12-stable HEK293 cells than in the control. The relative levels of KPNA6 are shown below the images. (C) Lower KPNA6 polyubiquitination in the HEK293 cells transfected with nsp12 plasmid. The MG132 treatment was done before the cell harvesting. KPNA6 IP, followed by WB with an antibody against ubiquitin (Ub), was performed. The relative levels of Ub are shown below the images. WB of WCL with the antibodies against Ub, KPNA6, PRRSV nsp12, and tubulin was conducted.