FIG 4.

KPNA6 silencing leads to lower replication of PRRSV. (A) KPNA6 protein level in the MARC-145 cells stably transfected with KPNA6-shRNA. CT, control shRNA; K6, shRNA against KPNA6. The WB analyses of KPNA6, KPNA1, and GAPDH were conducted. (B) Relative viability of shRNA-transfected MARC-145 cells. (C) Lower PRRSV yield in cells with KPNA6 silencing than in control MARC-145 cells (**, P < 0.01). MARC-145 cells were infected with PRRSV VR-2385 at an MOI of 0.1. The virus was harvested at 48 hpi for titration.