TABLE 1.

Primers used in this study

Primera	Sequence (5′–3′)b	Target gene/use
KPNA6F1	CCGAATTCGAGACCATGGCGAGCCCAGGG	KPNA6 cloning
KPNA6R1	GCCTCGAGTTATAGCTGGAAGCCCTCC	KPNA6 cloning
R-KPNA6-F1	GGAAGCCTATGGCTTGGATA	KPNA6 real-time
R-KPNA6-R1	CTGTTGCGTTTCATCGACTT	KPNA6 real-time
P7RF1	ATCGCTCACCAAAACCAGTCC	PRRSV real-time
P7RR1	CTTCAGTCGCTAGAGGGAAATGG	PRRSV real-time
KPNA6_S1F	GATCCAGCCTATGGCTTGGATAAATTCAAGAGATTTATCCAAGCCATAGGCTTTTTTTACGCGTG	KPNA6 shRNA
KPNA6_S1R	AATTCACGCGTAAAAAAAGCCTATGGCTTGGATAAATCTCTTGAATTTATCCAAGCCATAGGCTG	KPNA6 shRNA
KPNA6-gRNA-p2tF	CACCGAAGAGTGGTGGATCGGTTCG	KPNA6 sgRNA
KPNA6-gRNA-p2tR	AAACCGAACCGATCCACCACTCTTC	KPNA6 sgRNA
KPNA6-gRNA-p2bF	CACCGTGTTGATAACTTCATCTAT	KPNA6 sgRNA
KPNA6-gRNA-p2bR	AAACATAGATGAAGTTATCAACAC	KPNA6 sgRNA

^aF, forward primer; R, reverse primer.

^bItalicized letters indicate restriction enzyme cleavage sites for cloning.