FIG 2.

WDR5 overexpression enhances HCMV genome replication, viral gene expression, and infectious virus yield. HELFs were transduced with lentiviruses designed to overexpress WDR5 (OE) or the empty vector control (NC) and then infected with HCMV or HSV-1 at the indicated MOIs. Infected cells and culture supernatants were collected at the times indicated and analyzed to determine viral genome copy number, viral protein level, and infectious virus titers. (A) Viral genome replication. The HCMV genome copy number was determined by qPCR and normalized to the amount of cellular DNA (GAPDH). Results are shown as means ± SDs. (B) IB was used to determine relative WDR5 or viral protein levels in cell lysates. Representative images from three independent experiments are shown. (C) Growth kinetics of viral infection. NC and OE cells were infected with HCMV at an MOI of 3 (left) or 0.5 (middle) or HSV-1 at an MOI of 3 (right). Supernatant samples were collected at the indicated times postinfection, and infectious virus was titrated by a plaque formation assay. The average results from three independent ones are shown. Significance was analyzed by one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.