FIG 6.

Knockdown of WDR5 affects capsid nuclear egress by impairing NEC formation. Ctl, KD, or Rec cells were infected with HCMV at an MOI of 1, and the cells were fixed at the indicated times for IFA. (A) MCP (cyan) and pUL53 (red) were determined by IFA, and nuclei were counterstained with DAPI (blue). Representative images from three independent experiments are shown. (B) Cells staining positive for MCP or pUL53 were counted in 20 random fields. The percentages of pUL53-positive cells among MCP-positive populations during the course of HCMV infection are shown. Data from three independent experiments were analyzed by one-way ANOVA followed by the Bonferroni post hoc test. The results are presented as the means ± SDs. NS, not significant; *, P < 0.0167; **, P < 0.0033; ***, P < 0.0003. (C) Average numbers of pUL53 puncta per nuclear rim at 120 hpi in Ctl, KD, or Rec cells. Data were collected from three independent experiments and analyzed by the Kruskal-Wallis test followed by post hoc Dunn's multiple-comparison test. The medians from each experiment are indicated to the right of each plot, and the means from the three experiments for each cell line are shown above the data. NS, not significant; ***, P < 0.001.