FIG 7.

pUL50 and pUL53 colocalize in transfected Ctl, KD, or Rec cells. Uninfected Ctl, KD, or Rec cells were transiently transfected with plasmids carrying UL50-DsRed or hemagglutinin (HA)-UL53. Cells were fixed at 48 h posttransfection and stained with a mouse antihemagglutinin primary antibody, followed by Alexa Fluor 488-conjugated goat anti-mouse immunoglobulin secondary antibody (green). Nuclei were counterstained with DAPI (blue). Cells in the upper two rows were transfected with each plasmid independently, while cells in the next three rows were cotransfected with both plasmids simultaneously. Areas magnified in the bottom panels are framed by boxes in the panels above. Bars, 10 μm.