TABLE 1.
Frequencies of mutations in cell-free virus within the E2 region
| WT residue | Mutation(s) (frequency [%]) at passage:a |
|||
|---|---|---|---|---|
| 0 | 4 | 7 | 9 | |
| V414 | V (100) | V (100) | V (99), A (<1) | V (99), A (<1) |
| N415 | N (100) | N (100) | N (70), D (27), S (1), T (<1), G (<1) | D (79), N (16), S (3), T (<1), G (<1) |
| N417 | N (100) | N (99), S (<1) | N (98), S (1) | N (87), S (12) |
| G418 | G (100) | G (100) | G (99), A (<1) | G (99), A (<1) |
| I422 | I (100) | I (100) | I (100) | I (99), V (<1) |
| D431 | D (100) | D (100) | D (99), G (<1) | D (100) |
| I438 | I (100) | I (100) | I (100) | I (99), V (<1) |
| Y443 | Y (100) | Y (99), H (<1) | Y (100) | Y (100) |
| F447 | F (100) | F (100) | F (100) | F (99), L (<1) |
| M456 | M (100) | M (99), V (<1) | M (100) | M (100) |
| E482 | E (100) | E (100) | E (100) | E (99), G (<1) |
| N534 | N (100) | N (100) | N (99), S (<1) | N (99), D (<1) |
| L540 | L (100) | L (98), P (1), V (<1), I (<1) | L (99), P (<1), V (<1) | L (99), P (<1), V (<1) |
| L541 | L (100) | L (100) | L (99), S (<1) | L (100) |
a
Extracellular virus was obtained from the selected passage numbers from cultures of Huh7.5 cells initially infected with virus in the presence of 9.8 μg/ml MAb24 with serial passage of infected cells and extracellular virus.