Plaque sizes of PrV gH mutants. RK13 cells were infected with the indicated PrV recombinants and subsequently incubated under plaque assay conditions. After 48 h, areas of 30 plaques per virus were measured. Plaque areas of wild-type gH-expressing pPrV-gHK were set as 100%. Mean values and standard deviations from four independent experiments are shown. Results significantly differing from those obtained with pPrV-gHK are marked (*, P ≤ 0.05; **, P ≤ 0.01, ***, P ≤ 0.001). NS, not significant.