FIG 2.

Expression and surface localization of gH. RK13 cells were cotransfected with expression plasmids for either wild-type (WT) or mutant gH in combination with gL. After 24 h, cells were either permeabilized or not and were stained with a gH-specific rabbit antiserum and Alexa Fluor 488-conjugated secondary antibodies. Total and cell surface expression of gH was analyzed either by fluorescence microscopy (Nikon Eclipse Ti-S) (A) or by flow cytometry (B). Bars represent the ratio between cells expressing gH at the surface and total gH-positive cells. Mean values and standard deviations from three independent experiments are shown, and the significance of differences from results obtained with wild-type gH is marked. (**, P ≤ 0.01). Size bar in panel A, 100 μm.