FIG 3.

Subcellular localization of gH. RK13 cells were cotransfected with plasmids encoding wild-type (WT) or mutated gH in combination with gL. One day after transfection, cells were fixed with 3% paraformaldehyde and permeabilized with 0.1% Triton X-100. gH was detected using a monospecific rabbit antiserum and Alexa Fluor 488-conjugated secondary antibodies. Green fluorescence was excited at 488 nm and recorded with a laser scanning confocal microscope (Leica SP5). Size bar, 10 μm.