Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis of N-linked carbohydrates in gH. Purified virion proteins of the indicated PrV recombinants were either left untreated (lanes 1), digested with PNGase F (lanes 2) or endo H (lanes 3), or incubated with reaction buffer without enzymes (lanes 4). Samples were separated by SDS-PAGE, and blots were tested with a gH-specific rabbit antiserum. Molecular masses of marker proteins are indicated. Arrows indicate gH mutants exhibiting reduced or no endo H sensitivity.
