FIG 8.

Penetration kinetics of PrV gH mutants. RK13 cells grown in 6-well plates were infected with approximately 250 PFU/well of pPrV-gHK or the different gH mutants. After adsorption at 4°C, the cells were incubated for 0, 5, 10, 15, 30, 60, and 120 min at 37°C, followed by acid inactivation of nonpenetrated virus. After 48 h at 37°C under semisolid medium, plaques were counted and compared to plaque numbers found without inactivation. Mean percentages from three independent experiments and corresponding standard deviations are shown.