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In vitro growth kinetics of PrV gH mutants. RK13 cells were infected with pPrV-gHK or the different gH mutants at an MOI of 0.01. Cells and supernatants were harvested after 0, 6, 12, 24, 48, and 72 h at 37°C and lysed by freeze-thawing. Progeny virus titers were determined on RK13 cells. Shown are mean values from four independent experiments and corresponding standard deviations.
