TABLE 1.

Oligonucleotide primers for mutagenesis, PCR, and sequence analyses^a

Primer name	Sequence (5′ to 3′)b	Nucleotide positionsc
PGHN77Q-F	CTGGGGGCGCTCCAGGACACGCGCATC	60986–61012
PGHN162Q-F	GCGGCCGTCTTCCAGGTGACGCTGGGC	61241–61267
PGHN542Q-F	GCCATCGTCAGCCAGGACAGCGCCGCG	62381–62407
PGHN604Q-F	CATGGCCGGCGCCCAGTCCACCATCCC	62566–62592
PGHN627Q-F	TGATGCTCTTCCCCCAAGGCACCGTGGTC	62634–62662
PgH-PSF	TTCACGTCGGAGATGGGG	60611–60628
PgH-PSF2	GGAAGCCCTTCGACCAG	61875–61891
PgHK-PSR	CGCGAGCCCATTTATACCC	(Kanr)
PgH-PSR2	GTCGAGCAGGCTGAAGG	62055–62071 (r)
PgH-WH3	TGCACGAGAGCGACGACTACC	61479–61499

^aOnly forward-strand mutagenesis primers (F) are listed, since the reverse-strand primers were exactly complementary.

^bNonmatching nucleotides are shown in boldface.

^cNucleotide positions in the PrV-Ka genome refer to GenBank accession number JQ809328 (66), and a reverse-strand sequencing primer is indicated (r). PgHK-PSR binds to the inserted kanamycin resistance gene (Kan^r) of the PrV recombinants.