FIG 4.

rsT3D elicits more NF-κB activation than rsT1L in SVECs. SVECs were left untreated (U) or were treated with TNF-α (TNF) for 15 min, mock infected (M), or infected with rsT1L or rsT3D at an MOI of 100 PFU/cell. Whole-cell lysates were prepared at the indicated times, and immunoblot analysis was performed to detect phosphorylated IKKα/β (p-IKKα/β), IκBα, β-actin, or reovirus proteins. (B) SVECs were mock infected, infected with rsT1L or rsT3D at an MOI of 100 PFU/cell, or transfected with poly(I·C). At the indicated times, cytoplasmic and nuclear fraction lysates were prepared and immunoblot analysis was performed to detect p65, β-actin, α-tubulin, lamin A/C, or reovirus proteins. (C) Wild-type SVECs or MAVS-CRISPR SVECs were mock infected (M), treated with TNF-α (TNF) for 15 min, or infected with rsT1L or rsT3D at an MOI of 100 PFU/cell. Whole-cell lysates were prepared at the indicated times, and immunoblot analysis was performed to detect phosphorylated IKKα/β (p-IKKα/β), total IKKα/β, IκBα, MAVS, β-actin, or reovirus proteins.