FIG 5.

Viral protein synthesis and RNA synthesis are not required for reovirus-induced IRF3 phosphorylation. (A) SVECs were mock infected (M) or infected with rsT1L or rsT3D at an MOI of 100 PFU/cell in the absence or presence of ribavirin at the indicated concentrations. At 6 h, whole-cell lysates were prepared and immunoblot analysis was performed for phosphorylated-IRF3 (p-IRF3), total IRF3, β-actin, or reovirus proteins. (B) SVECs were infected with rsT1L or rsT3D at an MOI of 100 PFU/cell in the absence or presence of 40 μM ribavirin. At 6 h, total RNA was extracted and reovirus S4 mRNA levels were determined by RT-qPCR. Results are presented as the mean of triplicate samples ± SD and are representative of three independent experiments. *, P < 0.05; **, P < 0.01 (as determined by Student's t test).